Journal: Frontiers in Neuroscience

Article Title: A novel versatile hybrid infusion-multielectrode recording (HIME) system for acute drug delivery and multisite acquisition of neuronal activity in freely moving mice

doi: 10.3389/fnins.2015.00425

Figure Lengend Snippet: Construction details for electrodes .

Article Snippet: Once this was done, all the Tungsten wire tails from electrodes, starting from the longest tail (corresponding to the most superficially located electrode) were gently inserted into channels holes on the EIB-16, and pinned by golden pins (Neuralynx).

Techniques:

Journal: Frontiers in Neuroscience

Article Title: A novel versatile hybrid infusion-multielectrode recording (HIME) system for acute drug delivery and multisite acquisition of neuronal activity in freely moving mice

doi: 10.3389/fnins.2015.00425

Figure Lengend Snippet: Surgery procedure of implantation of hippocampal electrodes and bilateral injection guide cannulas in the mouse brain . Coordinates of implantation and position of ground (Gr.) and reference (Ref.) electrodes (A) . A scheme of implanted hippocampal multilayer electrode array and guide cannulas (B) . Surgery steps (C) . Neuralynx electrode-interface board (EIB-16) for 16 channels (D) . Construction of a protective mouse hat and a Faraday cage around electrodes, cannulas and EIB-16 (E) . The way how to handle and implant electrodes and cannulas by using tweezers and a metal ring (F) . Implantation setup connected to a preamplifier and a tether (G) .

Article Snippet: Once this was done, all the Tungsten wire tails from electrodes, starting from the longest tail (corresponding to the most superficially located electrode) were gently inserted into channels holes on the EIB-16, and pinned by golden pins (Neuralynx).

Techniques: Injection

Journal: Frontiers in Neuroscience

Article Title: A novel versatile hybrid infusion-multielectrode recording (HIME) system for acute drug delivery and multisite acquisition of neuronal activity in freely moving mice

doi: 10.3389/fnins.2015.00425

Figure Lengend Snippet: Quality of LFPs (A,C) and unit (C) recordings with the multielectrode (e.1–e.5) array targeting 5 different layers of the hippocampus . St. Or.-Pyr. Stratum Oriens Pyramidale, St. Rad. Stratum Radiatum, St. LM. Stratum Lacunosum-Moleculare, St. M.DG. Stratum Moleculare of Dentate Gyrus, St. Gr. Stratum Granulare . (B) An example of histological verification of electrode position in the dorsal right hippocampus, Nissl staining. (D) Verification of quality and power of oscillations after injection of a vehicle by analysis of signals at the first and fifth electrodes after the injection. Intrahippocampal bilateral injection of a vehicle did not influence power and frequency of θ-, β-, and γ-oscillations. (E) Power spectra density (dB) of hippocampal oscillations recorded before and after MK801 (0.5 μg/site) intrahippocampal injection. (F) Normalized power spectra densities and statistical evaluation for different frequency bands and hippocampal layers after MK801 injection. #### p < 0.001, Two-way ANOVA with post hoc Fisher LSD, * p < 0.05, ** p < 0.01, *** p < 0.005.

Article Snippet: Once this was done, all the Tungsten wire tails from electrodes, starting from the longest tail (corresponding to the most superficially located electrode) were gently inserted into channels holes on the EIB-16, and pinned by golden pins (Neuralynx).

Techniques: Staining, Injection

Journal: Frontiers in Neuroscience

Article Title: A novel versatile hybrid infusion-multielectrode recording (HIME) system for acute drug delivery and multisite acquisition of neuronal activity in freely moving mice

doi: 10.3389/fnins.2015.00425

Figure Lengend Snippet: Design and construction of the CA1/CA2 hippocampal multielectrode arrays of different complexity (C), implantation coordinates for cannulas and electrodes (A), quality of recording from 6 brain areas simultaneously (D) and behavior evaluation of implanted and injected with a vehicle mice in a sensitive novel object recognition task (B,E) . Obviously, animals explore a novel (N) object more than a familiar (F) one on day 1 (d1) and day 14 (d14) with standard novel object exploration time for this test (30–40 s during 10 min in OF), distance traveled (~30–40 m) and running speed (0.04 m/s). #### p < 0.001, Two-way ANOVA with post hoc Fisher LSD, * p < 0.05, *** p < 0.005.

Article Snippet: Once this was done, all the Tungsten wire tails from electrodes, starting from the longest tail (corresponding to the most superficially located electrode) were gently inserted into channels holes on the EIB-16, and pinned by golden pins (Neuralynx).

Techniques: Injection

Journal: Frontiers in Neuroscience

Article Title: A novel versatile hybrid infusion-multielectrode recording (HIME) system for acute drug delivery and multisite acquisition of neuronal activity in freely moving mice

doi: 10.3389/fnins.2015.00425

Figure Lengend Snippet: Parameters of the developed infusion-recording system .

Article Snippet: Once this was done, all the Tungsten wire tails from electrodes, starting from the longest tail (corresponding to the most superficially located electrode) were gently inserted into channels holes on the EIB-16, and pinned by golden pins (Neuralynx).

Techniques: Injection

Journal: PLoS ONE

Article Title: Characterization of Phase Transition in the Thalamocortical System during Anesthesia-Induced Loss of Consciousness

doi: 10.1371/journal.pone.0050580

Figure Lengend Snippet: Electrode placements in ventral posteromedial (A) and ventral lateral (B) nuclei were histologically verified with light microscopy. Arrow point indicates the tip of electrode. fi, fimbria of the hippocampus; st, stria terminalis; ic, internal capsule; cp, cerebral peduncle (basal part); VPM, ventral posteromedial nucleus; VPL, central posterolateral nucleus; ZI, zona incerta; STh, subthalamic nucleus; Po, posterior thalamic nuclear group; VM, ventromedial thalamic nucleus; DG, dentate gyrus; CM, central medial nucleus; VL, ventral lateral nucleus; VP, ventral posteromedial and posterolateral nuclei; Rt, reticular thalamic nucleus; LV, lateral ventricle. Scale bar is 1 mm.

Article Snippet: In brief, mice under ketamine-xylazine anesthesia (120 and 6 mg/kg, respectively, i.p.) were implanted with two tungsten electrodes (Teflon-insulated tungsten wire 76.2/114.3 μm in bare/coated diameter, A-M Systems, Sequim, WA, USA) positioned at the ventral lateral (1.06 mm posterior, 1.1 mm lateral and 3.5 mm ventral to bregma) and ventral posteromedial nuclei (1.82 mm posterior, 1.5 mm lateral and 3.7 mm ventral to bregma) of the thalamus; two cortical screw electrodes (chrome-plated stainless steel 3 mm in length and 1 mm in diameter, Asia Bolt, Seoul, Korea) positioned at the primary motor (0.74 mm anterior, 1.5 mm lateral and 0 mm ventral to bregma) and primary somatosensory cortices (1.82 mm posterior, 3.0 mm lateral and 0 mm ventral to the bregma); and a ground electrode on the interparietal bone.

Techniques: Light Microscopy